Next Generation Sequencing Sample Preparation Market Size Worth USD 16.57 Billion by 2034 | CAGR: 15.02%

The next generation sequencing sample preparation market size is expected to reach USD 16.57 billion by 2034, according to a new study by Polaris Market Research. The report “Next Generation Sequencing Sample Preparation Market Share, Size, Trends, Industry Analysis Report By Product & Service (Instruments, Consumables, Services), By Workflow, By Application, By End Use, By Region; Market Forecast, 2025–2034” gives a detailed insight into current market dynamics and provides analysis on future market growth.

Next generation sequencing (NGS) sample preparation is a critical step in the sequencing workflow that involves processing DNA or RNA samples to make them compatible with high-throughput sequencing platforms. The preparation usually begins with nucleic acid extraction, where DNA or RNA is isolated from biological samples such as blood, tissue, or cells. The extracted material is then quantified and assessed for purity using spectrophotometry or fluorometry. For RNA sequencing (RNA-seq), an additional step of converting RNA into complementary DNA (cDNA) is required. The DNA or cDNA is then fragmented into smaller pieces, either enzymatically or mechanically, to facilitate sequencing.

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Next generation sequencing sample preparation is used across various applications, including whole genome sequencing, targeted DNA RNA sequencing, transcriptomics, epigenetics, and metagenomics. In clinical diagnostics, it enables the identification of genetic mutations linked to diseases, while in cancer research, it helps characterize tumor genomes. In infectious disease studies, NGS aids in pathogen detection and outbreak surveillance.

Advancements in library preparation kits and automated systems have streamlined the process, reducing hands-on time and improving reproducibility. NGS sample preparation is the foundation for generating high-quality sequencing data, enabling breakthroughs in genomics, personalized medicine, and biotechnology.

Top of FormNext Generation Sequencing Sample Preparation Market Report Highlights

• In terms of product & service, the consumables segment accounted for a major market share in 2024. Researchers and laboratories across clinical, academic, and pharmaceutical sectors heavily relied on reagents, kits, and other disposable materials due to the high frequency of sample preparation workflows.
• Based on workflow, the library preparation & amplification segment held the largest share of the next generation sequencing (NGS) sample preparation market in 2024 due to its crucial role in enabling accurate and high-throughput sequencing.
• In terms of application, the oncology segment held the largest market share in 2024 due to the ability of next generation sequencing sample preparation to uncover somatic mutations, identify tumor-specific biomarkers, and guide targeted therapies.
• Based on end use, the pharma & biotech entities segment accounted for a major market share in 2024. These organizations drove substantial demand for sequencing sample preparation due to their extensive use of NGS technologies in drug discovery, biomarker identification, and companion diagnostics.
• A few global key players in the NGS  sample preparation market include Agilent Technologies, Inc.; BGI Genomics; Bio-Rad Laboratories, Inc.; Danaher Corporation; Eurofins Scientific; F. Hoffmann-La Roche Ltd; Illumina, Inc.; PerkinElmer Inc.; QIAGEN; and Thermo Fisher Scientific Inc.

Polaris Market Research has segmented the next generation sequencing sample preparation market report on the basis of product & service, workflow, application, end use, and region:

By Product & Service Outlook (Revenue, USD Billion, 2020–2034)

• Instruments
• Consumables
• Services

By Workflow Outlook (Revenue, USD Billion, 2020–2034)

• Nucleic Acid Extraction
• Library Preparation & Amplification
• Target Enrichment
• Library Quantification/QC

By Application Outlook (Revenue, USD Billion, 2020–2034)

• Oncology
• Clinical Investigation
• Reproductive Health
• HLA Typing/Immune System Monitoring
• Metagenomics, Epidemiology & Drug Development
• Agrigenomics & Forensics
• Consumer Genomics

By End Use Outlook (Revenue, USD Billion, 2020–2034)

• Academic Research
• Clinical Research
• Hospitals & Clinics
• Pharma & Biotech Entities
• Other

By Regional Outlook (Revenue, USD Billion, 2020–2034)

• North America
  • US
  • Canada
• Europe
  • Germany
  • France
  • UK
  • Italy
  • Spain
  • Netherlands
  • Russia
  • Rest of Europe
• Asia Pacific
  • China
  • Japan
  • India
  • Malaysia
  • South Korea
  • Indonesia
  • Australia
  • Vietnam
  • Rest of Asia Pacific
• Middle East & Africa
  • Saudi Arabia
  • UAE
  • Israel
  • South Africa
  • Rest of Middle East & Africa
• Latin America
  • Mexico
  • Brazil
  • Argentina
  • Rest of Latin America